HRH3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HRH3 polyclonal antibody (Cat # PAB3659) in Jurkat cell line lysate (35 ug/lane). HH3R (arrow) was detected using the purified polyclonal antibody (1:1000 dilution). This western blot identifies isoform two of HRH3. The accession number of HRH3 is CAC39434 ; Q9Y5N1.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 polyclonal antibody (Cat # PAB3659) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HRH3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human HRH3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HRH3 polyclonal antibody (Cat # PAB3659) in Jurkat cell line lysate (35 ug/lane). HH3R (arrow) was detected using the purified polyclonal antibody (1:1000 dilution). This western blot identifies isoform two of HRH3. The accession number of HRH3 is CAC39434 ; Q9Y5N1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with HRH3 polyclonal antibody (Cat # PAB3659) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — HRH3
Entrez GeneID
11255Protein Accession#
BAB17030;Q9Y5N1-2Gene Name
HRH3
Gene Alias
GPCR97, HH3R
Gene Description
histamine receptor H3
Omim ID
604525Gene Ontology
HyperlinkGene Summary
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq
Other Designations
G protein-coupled receptor 97|OTTHUMP00000031459|histamine H3 receptor
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Interactome
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