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Last updated: 2016/12/4
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FREQ polyclonal antibody

  • Catalog # : PAB3601
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FREQ.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to internal region of human FREQ.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Flow Cytometry (1:10-50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of MCF-7 cell lysate (35 ug/lane) with FREQ polyclonal antibody (Cat # PAB3601).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human breast carcinomareacted with FREQ polyclonal antibody (Cat # PAB3601), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of ZR-75-1 cells using FREQ polyclonal antibody (Cat # PAB3601)(bottom histogram) compared to a negative control cell (top histogram).
    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • FREQ
  • Gene Alias:
  • DKFZp761L1223,FLUP,NCS-1,NCS1
  • Gene Description:
  • frequenin homolog (Drosophila)
  • Gene Summary:
  • This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022356,frequenin homolog,frequenin-like ubiquitous protein,neuronal calcium sensor 1
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