GJA9 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate (35 ug/lane) with GJA9 polyclonal antibody (Cat # PAB3599).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJA9 polyclonal antibody (Cat # PAB3599) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJA9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GJA9.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse heart tissue lysate (35 ug/lane) with GJA9 polyclonal antibody (Cat # PAB3599).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJA9 polyclonal antibody (Cat # PAB3599) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — GJA9
Entrez GeneID
81025Protein Accession#
NP_110399;P57773Gene Name
GJA9
Gene Alias
CX58, CX59, GJA10, MGC50985
Gene Description
gap junction protein, alpha 9, 59kDa
Gene Ontology
HyperlinkGene Summary
Connexins, such as GJA9, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM
Other Designations
OTTHUMP00000000542|connexin 59|gap junction alpha 10|gap junction protein, alpha 10, 59kDa
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Disease
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Publication Reference
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Functional expression of connexin57 in horizontal cells of the mouse retina.
Hombach S, Janssen-Bienhold U, Sohl G, Schubert T, Bussow H, Ott T, Weiler R, Willecke K.
The European Journal of Neuroscience 2004 May; 19(10):2633.
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Functional significance of gap junctional coupling in preimplantation development.
Houghton FD, Barr KJ, Walter G, Gabriel HD, Grummer R, Traub O, Leese HJ, Winterhager E, Kidder GM.
Biology of Reproduction 2002 May; 66(5):1403.
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Molecular cloning and functional expression of the mouse gap junction gene connexin-57 in human HeLa cells.
Manthey D, Bukauskas F, Lee CG, Kozak CA, Willecke K.
The Journal of Biological Chemistry 1999 May; 274(21):14716.
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Functional expression of connexin57 in horizontal cells of the mouse retina.
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