GJC1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of GJC1 polyclonal antibody (Cat # PAB3593) in A547 cell lysate. GJA7 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJC1 polyclonal antibody (Cat # PAB3593) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJC1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GJC1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of GJC1 polyclonal antibody (Cat # PAB3593) in A547 cell lysate. GJA7 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJC1 polyclonal antibody (Cat # PAB3593) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — GJC1
Entrez GeneID
10052Protein Accession#
P36383Gene Name
GJC1
Gene Alias
CX45, DKFZp686P0738, GJA7
Gene Description
gap junction protein, gamma 1, 45kDa
Omim ID
608655Gene Ontology
HyperlinkGene Summary
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Alternatively spliced transcript variants encoding the same isoform have been described. [provided by RefSeq
Other Designations
connexin 45|gap junction protein, alpha 7, 45kDa
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Interactome
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Disease
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Publication Reference
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[Expression of connexin43 and connexin45 in nasopharyngeal carcinoma].
Xiang Q, Fan SQ, Li J, Tan C, Xiang JJ, Zhang QH, Wang R, Li GY.
Ai Zheng 2002 Jun; 21(6).
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Comparison of connexin 43, 40 and 45 expression patterns in the developing human and mouse hearts.
Kaba RA, Coppen SR, Dupont E, Skepper JN, Elneil S, Haw MP, Pepper JR, Yacoub MH, Rothery S, Severs NJ.
Cell Adhesion and Communication 2001 Jan; 8(4-6):339.
Application：IF, IHC-Fr, Human, Mouse, Hearts.
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Molecular cloning of two human cardiac gap junction proteins, connexin40 and connexin45.
Kanter HL, Saffitz JE, Beyer EC.
Journal of Molecular and Cellular Cardiology 1994 Jul; 26(7):861.
Application：IF, IHC-Fr, WB-Ti, Human, Human hearts.
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[Expression of connexin43 and connexin45 in nasopharyngeal carcinoma].
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