GJB3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of WiDr cell lysate (35 ug/lane) with GJB3 polyclonal antibody (Cat # PAB3584) at 2 ug/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJB3 polyclonal antibody (Cat # PAB3584) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GJB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human GJB3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of WiDr cell lysate (35 ug/lane) with GJB3 polyclonal antibody (Cat # PAB3584) at 2 ug/mL.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJB3 polyclonal antibody (Cat # PAB3584) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — GJB3
Entrez GeneID
2707Protein Accession#
NP_076872;O75712Gene Name
GJB3
Gene Alias
CX31, DFNA2, EKV, FLJ22486, MGC102938
Gene Description
gap junction protein, beta 3, 31kDa
Gene Ontology
HyperlinkGene Summary
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000004183|OTTHUMP00000004184|connexin 31
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Interactomes
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Diseases
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Publication Reference
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Mutation analysis of Connexin 31 (GJB3) in sporadic non-syndromic hearing impairment.
Mhatre AN, Weld E, Lalwani AK.
Clinical Genetics 2003 Feb; 63(2):154.
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Expression of a connexin31 mutation causing erythrokeratodermia variabilis is lethal for HeLa cells.
Diestel S, Richard G, Doring B, Traub O.
Biochemical and Biophysical Research Communications 2002 Aug; 296(3):721.
Application：IF, WB-Tr, Human, HeLa cells.
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Defective trafficking and cell death is characteristic of skin disease-associated connexin 31 mutations.
Di WL, Monypenny J, Common JE, Kennedy CT, Holland KA, Leigh IM, Rugg EL, Zicha D, Kelsell DP.
Human Molecular Genetics 2002 Aug; 11(17):2005.
Application：IF, WB-Ce, Human, Skin.
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Mutation analysis of Connexin 31 (GJB3) in sporadic non-syndromic hearing impairment.
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