STAT5B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate (35 ug/lane) with STAT5B polyclonal antibody (Cat # PAB3577).
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with STAT5B polyclonal antibody (Cat # PAB3577). (Kindly provided by Dr. John Wu)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT5B polyclonal antibody (Cat # PAB3577) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of STAT5B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human STAT5B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate (35 ug/lane) with STAT5B polyclonal antibody (Cat # PAB3577).Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with STAT5B polyclonal antibody (Cat # PAB3577). (Kindly provided by Dr. John Wu)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT5B polyclonal antibody (Cat # PAB3577) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — STAT5B
Entrez GeneID
6777Protein Accession#
P51692Gene Name
STAT5B
Gene Alias
STAT5
Gene Description
signal transducer and activator of transcription 5B
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq
Other Designations
transcription factor STAT5B
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Interactome
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Pathway
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Disease
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Publication Reference
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Constitutive activation of Stat5b contributes to carcinogenesis in vivo.
Xi S, Zhang Q, Gooding WE, Smithgall TE, Grandis JR.
Cancer Research 2003 Oct; 63(20):6763.
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Role of Stat5 in type I interferon-signaling and transcriptional regulation.
Uddin S, Lekmine F, Sassano A, Rui H, Fish EN, Platanias LC.
Biochemical and Biophysical Research Communications 2003 Aug; 308(2):325.
Application：IP, WB, Human, Daudi, KG1 cells.
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Regulation of eosinophil apoptosis by nitric oxide: Role of c-Jun-N-terminal kinase and signal transducer and activator of transcription 5.
Zhang X, Msc, Moilanen E, Lahti A, Hamalainen M, Giembycz MA, Barnes PJ, Lindsay MA, Kankaanranta H.
The Journal of Allergy and Clinical Immunology 2003 Jul; 112(1):93.
Application：WB, Recombinant protein.
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Constitutive activation of Stat5b contributes to carcinogenesis in vivo.
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