Last updated: 2017/10/15

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STAT5B polyclonal antibody

Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human STAT5B.

Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.

Storage Instruction:
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.

Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

1.
Constitutive activation of Stat5b contributes to carcinogenesis in vivo.
Xi S, Zhang Q, Gooding WE, Smithgall TE, Grandis JR.Cancer Res. 2003 Oct 15;63(20):6763-71.

2.
Role of Stat5 in type I interferon-signaling and transcriptional regulation.
Uddin S, Lekmine F, Sassano A, Rui H, Fish EN, Platanias LC.Biochem Biophys Res Commun. 2003 Aug 22;308(2):325-30.

3.
Regulation of eosinophil apoptosis by nitric oxide: Role of c-Jun-N-terminal kinase and signal transducer and activator of transcription 5.
Zhang X, Msc, Moilanen E, Lahti A, Hamalainen M, Giembycz MA, Barnes PJ, Lindsay MA, Kankaanranta H.J Allergy Clin Immunol. 2003 Jul;112(1):93-101.

Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with STAT5B polyclonal antibody (Cat # PAB3577). (Kindly provided by Dr. John Wu)

Western Blot (Cell lysate)
Western blot analysis of Jurkat cell lysate (35 ug/lane) with STAT5B polyclonal antibody (Cat # PAB3577).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with STAT5B polyclonal antibody (Cat # PAB3577) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Gene Summary:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq