CDH3 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of CDH3 (arrow) using rabbit CDH3 polyclonal antibody (Cat # PAB3539) in K-562 cell line lysates (35 ug/lane). CDH3 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Western Blot (Transfected lysate)
Western blot analysis of CDH3 (arrow) using rabbit CDH3 polyclonal antibody (Cat # PAB3539). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with CDH3 polyclonal antibody (Cat # PAB3539) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CDH3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CDH3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CDH3 (arrow) using rabbit CDH3 polyclonal antibody (Cat # PAB3539) in K-562 cell line lysates (35 ug/lane). CDH3 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Western Blot (Transfected lysate)
Western blot analysis of CDH3 (arrow) using rabbit CDH3 polyclonal antibody (Cat # PAB3539). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDH3 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with CDH3 polyclonal antibody (Cat # PAB3539) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — CDH3
Entrez GeneID
1001Protein Accession#
NP_001784;P22223Gene Name
CDH3
Gene Alias
CDHP, HJMD, PCAD
Gene Description
cadherin 3, type 1, P-cadherin (placental)
Gene Ontology
HyperlinkGene Summary
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congential hypotrichosis with juvenile macular dystrophy. [provided by RefSeq
Other Designations
cadherin 3, type 1|calcium-dependent adhesion protein, placental
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Interactome
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Pathway
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Disease
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Publication Reference
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Novel CDH3 mutations in hypotrichosis with juvenile macular dystrophy.
Indelman M, Eason J, Hummel M, Loza O, Suri M, Leys MJ, Bayne M, Schwartz FL, Sprecher E.
Clinical and Experimental Dermatology 2007 Mar; 32(2):191.
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P-cadherin overexpression is an indicator of clinical outcome in invasive breast carcinomas and is associated with CDH3 promoter hypomethylation.
Paredes J, Albergaria A, Oliveira JT, Jeronimo C, Milanezi F, Schmitt FC.
Clinical Cancer Research 2005 Aug; 11(16):5869.
Application：IHC-P, WB-Ti, Human, Human breast carcinomas, MCF-7 cells.
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Novel CDH3 mutations in hypotrichosis with juvenile macular dystrophy.
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