TESK2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of TESK2 polyclonal antibody (Cat # PAB3502) in HL-60 cell line lysates (35 ug/lane). TESK2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 polyclonal antibody (Cat # PAB3502) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TESK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human TESK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TESK2 polyclonal antibody (Cat # PAB3502) in HL-60 cell line lysates (35 ug/lane). TESK2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human testis tissue reacted with TESK2 polyclonal antibody (Cat # PAB3502) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. -
Gene Info — TESK2
Entrez GeneID
10420Protein Accession#
NP_009101;Q96S53Gene Name
TESK2
Gene Alias
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Gene Description
testis-specific kinase 2
Omim ID
604746Gene Ontology
HyperlinkGene Summary
This gene product is a serine/threonine protein kinase that contains an N-terminal protein kinase domain that is structurally similar to the kinase domains of testis-specific protein kinase-1 and the LIM motif-containing protein kinases (LIMKs). Its overall structure is most related to the former, indicating that it belongs to the TESK subgroup of the LIMK/TESK family of protein kinases. This gene is predominantly expressed in testis and prostate. The developmental expression pattern of the rat gene in testis suggests an important role for this gene in meitoic stages and/or early stages of spermiogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000009093|testis-specific protein kinase 2
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Interactome
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Publication Reference
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Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells.
Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K.
The Journal of Biological Chemistry 2001 Aug; 276(33):31449.
Application：IHC-Fr, IP-WB, WB-Tr, Human, Dog, Monkey, Mouse, Rat, HeLa, COS-7, Rat1A, Swiss 3T3, MDCK, HL60, Jurkat cells, Testis.
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Identification and characterization of TESK2, a novel member of the LIMK/TESK family of protein kinases, predominantly expressed in testis.
Rosok O, Pedeutour F, Ree AH, Aasheim HC.
Genomics 1999 Oct; 61(1):44.
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Cofilin phosphorylation and actin reorganization activities of testicular protein kinase 2 and its predominant expression in testicular Sertoli cells.
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