RAF1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RAF1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of RAF1 polyclonal antibody (Cat # PAB3487) in Jurkat cell lysate. RAF1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RAF1 polyclonal antibody (Cat # PAB3487) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
Flow Cytometry
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Gene Info — RAF1
Entrez GeneID
5894Protein Accession#
P04049Gene Name
RAF1
Gene Alias
CRAF, NS5, Raf-1, c-Raf
Gene Description
v-raf-1 murine leukemia viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Other Designations
Oncogene RAF1|raf proto-oncogene serine/threonine protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of Raf through phosphorylation and N terminus-C terminus interaction.
Chong H, Guan KL.
The Journal of Biological Chemistry 2003 Jul; 278(38):36269.
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Role of Raf in vascular protection from distinct apoptotic stimuli.
Alavi A, Hood JD, Frausto R, Stupack DG, Cheresh DA.
Science 2003 Jul; 301(5629):94.
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Down-regulation of Raf-1 kinase is associated with paclitaxel resistance in human breast cancer MCF-7/Adr cells.
Lee M, Koh WS, Han SS.
Cancer Letters 2003 Apr; 193(1):57.
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Regulation of Raf through phosphorylation and N terminus-C terminus interaction.
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