Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human RAF1.
Protein G purification
Flow Cytometry (1:10-50) Immunofluorescence (1:10-50) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In PBS (0.09% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of RAF1 polyclonal antibody (Cat # PAB3487) in Jurkat cell lysate. RAF1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with RAF1 polyclonal antibody (Cat # PAB3487) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
Oncogene RAF1,raf proto-oncogene serine/threonine protein kinase