PTK7 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of CCK4 (arrow) using rabbit PTK7 polyclonal antibody (Cat # PAB3464). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PTK7 polyclonal antibody (Cat # PAB3464) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Flow Cytometry
Flow cytometric analysis of NCI-H460 cells using PTK7 polyclonal antibody (Cat # PAB3464)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PTK7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PTK7.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Transfected lysate)
Western blot analysis of CCK4 (arrow) using rabbit PTK7 polyclonal antibody (Cat # PAB3464). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PTK7 polyclonal antibody (Cat # PAB3464) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.Flow Cytometry
Flow cytometric analysis of NCI-H460 cells using PTK7 polyclonal antibody (Cat # PAB3464)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — PTK7
Entrez GeneID
5754Protein Accession#
Q13308Gene Name
PTK7
Gene Alias
CCK4
Gene Description
PTK7 protein tyrosine kinase 7
Omim ID
601890Gene Ontology
HyperlinkGene Summary
Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000039809|colon carcinoma kinase-4
-
Interactome
-
Publication Reference
-
Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
Zhang H, Li XJ, Martin DB, Aebersold R.
Nature Biotechnology 2003 Jun; 21(6):660.
-
Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG.
Park SK, Lee HS, Lee ST.
Journal of Biochemistry 1996 Feb; 119(2):235.
-
Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family.
Mossie K, Jallal B, Alves F, Sures I, Plowman GD, Ullrich A.
Oncogene 1995 Nov; 11(10).
-
Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com