Western blot analysis of CCK4 (arrow) using rabbit PTK7 polyclonal antibody (Cat # PAB3464). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PTK7 polyclonal antibody (Cat # PAB3464) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Flow cytometric analysis of NCI-H460 cells using PTK7 polyclonal antibody (Cat # PAB3464)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq