FER polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FER.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 789-818 at C-terminus of human FER.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The FER polyclonal antibody (Cat # PAB3423) is used in Western blot to detect FER in HL-60 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Flow Cytometry
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Gene Info — FER
Entrez GeneID
2241Protein Accession#
P16591Gene Name
FER
Gene Alias
TYK3
Gene Description
fer (fps/fes related) tyrosine kinase
Omim ID
176942Gene Ontology
HyperlinkGene Summary
Fer protein is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. [provided by RefSeq
Other Designations
phosphoprotein NCP94
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Interactome
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Pathway
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Disease
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Publication Reference
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Closing in on the biological functions of Fps/Fes and Fer.
Greer P.
Nature Reviews. Molecular Cell Biology 2002 Apr; 3(4):278.
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Involvement of the tyrosine kinase fer in cell adhesion.
Rosato R, Veltmaat JM, Groffen J, Heisterkamp N.
Molecular and Cellular Biology 1998 Oct; 18(10):5762.
Application:IP-WB, WB-Ce, Rat, Fibroblasts.
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Growth factor-dependent phosphorylation of the actin-binding protein cortactin is mediated by the cytoplasmic tyrosine kinase FER.
Kim L, Wong TW.
The Journal of Biological Chemistry 1998 Sep; 273(36):23542.
Application:IF, WB-Ce, WB-Tr, Human, Mouse, NIH/3T3, 293 cells.
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Closing in on the biological functions of Fps/Fes and Fer.
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