BTK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The BTK polyclonal antibody (Cat # PAB3419) is used in Western blot to detect BTK in Ramos cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of BTK polyclonal antibody (Cat # PAB3419) transiently transfected HEK293 cell line lysate (1 ug/lane). BTK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BTK polyclonal antibody (Cat # PAB3419) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BTK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 209-239 at internal region of human BTK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Saturated ammonium sulfate precipitation
Recommend Usage
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C for up to 2 weeks. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The BTK polyclonal antibody (Cat # PAB3419) is used in Western blot to detect BTK in Ramos cell lysate.Western Blot (Transfected lysate)
Western blot analysis of BTK polyclonal antibody (Cat # PAB3419) transiently transfected HEK293 cell line lysate (1 ug/lane). BTK polyclonal antibody (arrow) was detected using the purified polyclonal antibody (1 : 1500 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with BTK polyclonal antibody (Cat # PAB3419) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
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Gene Info — BTK
Entrez GeneID
695Protein Accession#
Q06187Gene Name
BTK
Gene Alias
AGMX1, AT, ATK, BPK, IMD1, MGC126261, MGC126262, PSCTK1, XLA
Gene Description
Bruton agammaglobulinemia tyrosine kinase
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq
Other Designations
B-cell progenitor kinase|Bruton tyrosine kinase|OTTHUMP00000023676|OTTHUMP00000063593|agammaglobulinemia tyrosine kinase|dominant-negative kinase-deficient Brutons tyrosine kinase|tyrosine-protein kinase BTK
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Interactome
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Pathway
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Disease
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Publication Reference
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Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering.
Marquez JA, Smith CI, Petoukhov MV, Lo Surdo P, Mattsson PT, Knekt M, Westlund A, Scheffzek K, Saraste M, Svergun DI.
The EMBO Journal 2003 Sep; 22(18):4616.
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Bruton's tyrosine kinase is required for lipopolysaccharide-induced tumor necrosis factor alpha production.
Horwood NJ, Mahon T, McDaid JP, Campbell J, Mano H, Brennan FM, Webster D, Foxwell BM.
The Journal of Experimental Medicine 2003 Jun; 197(12):1603.
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Bruton's tyrosine kinase is a Toll/interleukin-1 receptor domain-binding protein that participates in nuclear factor kappaB activation by Toll-like receptor 4.
Jefferies CA, Doyle S, Brunner C, Dunne A, Brint E, Wietek C, Walch E, Wirth T, O'Neill LA.
The Journal of Biological Chemistry 2003 Jul; 278(28):26258.
Application：IP, WB-Tr, Human, HEK 293, THP-1 cells.
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Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering.
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