TNK2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of TNK2 polyclonal antibody (Cat # PAB3416) in CEM cell line lysates (35 ug/lane). TNK2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TNK2 polyclonal antibody (Cat # PAB3416) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TNK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TNK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TNK2 polyclonal antibody (Cat # PAB3416) in CEM cell line lysates (35 ug/lane). TNK2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TNK2 polyclonal antibody (Cat # PAB3416) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — TNK2
Entrez GeneID
10188Protein Accession#
Q07912Gene Name
TNK2
Gene Alias
ACK, ACK1, FLJ44758, FLJ45547, p21cdc42Hs
Gene Description
tyrosine kinase, non-receptor, 2
Omim ID
606994Gene Ontology
HyperlinkGene Summary
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq
Other Designations
activated Cdc42-associated kinase 1|activated p21cdc42Hs kinase
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Interactome
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Publication Reference
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Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck.
Yokoyama N, Miller WT.
The Journal of Biological Chemistry 2003 Sep; 278(48):47713.
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A non-receptor tyrosine kinase that inhibits the GTPase activity of p21cdc42.
Manser E, Leung T, Salihuddin H, Tan L, Lim L.
Nature 1993 May; 363(6427):364.
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Biochemical properties of the Cdc42-associated tyrosine kinase ACK1. Substrate specificity, authphosphorylation, and interaction with Hck.
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