TEK polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TEK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TEK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of TEK (arrow) using rabbit TEK polyclonal antibody (Cat # PAB3395). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TEK gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TEK polyclonal antibody (Cat # PAB3395) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — TEK
Entrez GeneID
7010Protein Accession#
Q02763Gene Name
TEK
Gene Alias
CD202B, TIE-2, TIE2, VMCM, VMCM1
Gene Description
TEK tyrosine kinase, endothelial
Gene Ontology
HyperlinkGene Summary
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq
Other Designations
OTTHUMP00000021167|soluble TIE2 variant 1|soluble TIE2 variant 2
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Interactome
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Disease
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Publication Reference
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Tie2 receptor tyrosine kinase, a major mediator of tumor necrosis factor alpha-induced angiogenesis in rheumatoid arthritis.
DeBusk LM, Chen Y, Nishishita T, Chen J, Thomas JW, Lin PC.
Arthritis and Rheumatism 2003 Sep; 48(9):2461.
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Expression of Tie-2 in human peripheral and autonomic nervous system.
Poncet S, Gasc JM, Janzer RC, Meyer S, Juillerat-Jeanneret L.
Neuropathology and Applied Neurobiology 2003 Aug; 29(4):361.
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Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1.
Cascone I, Audero E, Giraudo E, Napione L, Maniero F, Philips MR, Collard JG, Serini G, Bussolino F.
Blood 2003 Oct; 102(7):2482.
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Tie2 receptor tyrosine kinase, a major mediator of tumor necrosis factor alpha-induced angiogenesis in rheumatoid arthritis.
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