ROR2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of ROR2 (arrow) using ROR2 polyclonal antibody (Cat # PAB3385).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR2 gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human kidney carcionmareacted with ROR2 polyclonal antibody (Cat # PAB3385), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using ROR2 polyclonal antibody (Cat # PAB3385)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ROR2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ROR2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of ROR2 (arrow) using ROR2 polyclonal antibody (Cat # PAB3385).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR2 gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human kidney carcionmareacted with ROR2 polyclonal antibody (Cat # PAB3385), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using ROR2 polyclonal antibody (Cat # PAB3385)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ROR2
Entrez GeneID
4920Protein Accession#
Q01974Gene Name
ROR2
Gene Alias
BDB, BDB1, MGC163394, NTRKR2
Gene Description
receptor tyrosine kinase-like orphan receptor 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. [provided by RefSeq
Other Designations
OTTHUMP00000021634|OTTHUMP00000063680|neurotrophic tyrosine kinase receptor-related 2|tyrosine-protein kinase transmembrane receptor ROR2
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Interactome
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Disease
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Publication Reference
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Expression of Wnt5a and ROR2, Components of the Noncanonical Wnt-Signaling Pathway, is Associated with Tumor Differentiation in Hepatocellular Carcinoma.
Kazuki Wakizaka, Toshiya Kamiyama, Tatsuhiko Kakisaka, Tatsuya Orimo, Akihisa Nagatsu, Takeshi Aiyama, Shunsuke Shichi, Akinobu Taketomi.
Annals of Surgical Oncology 2023 Oct; [Epub].
Application：IHC, WB, Human, Human hepatocellular carcinoma tissue, HepG2, Huh7 cells.
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Mutation of the gene encoding the ROR2 tyrosine kinase causes autosomal recessive Robinow syndrome.
van Bokhoven H, Celli J, Kayserili H, van Beusekom E, Balci S, Brussel W, Skovby F, Kerr B, Percin EF, Akarsu N, Brunner HG.
Nature Genetics 2000 Aug; 25(4):423.
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Recessive Robinow syndrome, allelic to dominant brachydactyly type B, is caused by mutation of ROR2.
Afzal AR, Rajab A, Fenske CD, Oldridge M, Elanko N, Ternes-Pereira E, Tuysuz B, Murday VA, Patton MA, Wilkie AO, Jeffery S.
Nature Genetics 2000 Aug; 25(4):419.
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Dominant mutations in ROR2, encoding an orphan receptor tyrosine kinase, cause brachydactyly type B.
Oldridge M, Fortuna AM, Maringa M, Propping P, Mansour S, Pollitt C, DeChiara TM, Kimble RB, Valenzuela DM, Yancopoulos GD, Wilkie AO.
Nature Genetics 2000 Mar; 24(3):275.
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Expression of Wnt5a and ROR2, Components of the Noncanonical Wnt-Signaling Pathway, is Associated with Tumor Differentiation in Hepatocellular Carcinoma.
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