INSR polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of INSR.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human INSR.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of INSR polyclonal antibody (Cat # PAB3356) in SK-BR-3 cell lysate. INSR (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with INSR polyclonal antibody (Cat # PAB3356) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — INSR
Entrez GeneID
3643Protein Accession#
P06213Gene Name
INSR
Gene Alias
CD220, HHF5
Gene Description
insulin receptor
Gene Ontology
HyperlinkGene Summary
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject.
George S, Johansen A, Soos MA, Mortensen H, Gammeltoft S, Saudek V, Siddle K, Hansen L, O'Rahilly S.
Endocrinology 2003 Feb; 144(2):631.
Application:IP, WB-Tr, Mouse, CHO cells.
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Genotype-phenotype correlation in inherited severe insulin resistance.
Longo N, Wang Y, Smith SA, Langley SD, DiMeglio LA, Giannella-Neto D.
Human Molecular Genetics 2002 Jun; 11(12):1465.
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An arginine to cysteine(252) mutation in insulin receptors from a patient with severe insulin resistance inhibits receptor internalisation but preserves signalling events.
Hamer I, Foti M, Emkey R, Cordier-Bussat M, Philippe J, De Meyts P, Maeder C, Kahn CR, Carpentier JL.
Diabetologia 2002 Apr; 45(5):657.
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Deletion of V335 from the L2 domain of the insulin receptor results in a conformationally abnormal receptor that is unable to bind insulin and causes Donohue's syndrome in a human subject.
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