DGKA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate (35 ug/lane) with DGKA polyclonal antibody (Cat # PAB3350).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKA polyclonal antibody (Cat # PAB3350) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DGKA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DGKA.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HL-60 cell lysate (35 ug/lane) with DGKA polyclonal antibody (Cat # PAB3350).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKA polyclonal antibody (Cat # PAB3350) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — DGKA
Entrez GeneID
1606Protein Accession#
NP_958853;P23743Gene Name
DGKA
Gene Alias
DAGK, DAGK1, DGK-alpha, MGC12821, MGC42356
Gene Description
diacylglycerol kinase, alpha 80kDa
Omim ID
125855Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
diacylglycerol kinase alpha|diacylglycerol kinase, alpha (80kD)
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Interactome
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Pathway
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Publication Reference
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Assignment of the human diacylglycerol kinase gene (DAGK) to 12q13.3 using fluorescence in situ hybridization analysis.
Hart TC, Zhou J, Champagne C, Van Dyke TE, Rao PN, Pettenati MJ.
Genomics 1994 Jul; 22(1):246.
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Assignment of the gene for diacylglycerol kinase (DAGK) to human chromosome 12.
Hart TC, Champagne C, Zhou J, Van Dyke TE.
Mammalian Genome 1994 Feb; 5(2):123.
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Purification, cDNA-cloning and expression of human diacylglycerol kinase.
Schaap D, de Widt J, van der Wal J, Vandekerckhove J, van Damme J, Gussow D, Ploegh HL, van Blitterswijk WJ, van der Bend RL.
FEBS Letters 1990 Nov; 275(1-2):151.
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Assignment of the human diacylglycerol kinase gene (DAGK) to 12q13.3 using fluorescence in situ hybridization analysis.
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