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Last updated: 2016/12/4
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DGKA polyclonal antibody

  • Catalog # : PAB3350
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of DGKA.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DGKA.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of HL-60 cell lysate (35 ug/lane) with DGKA polyclonal antibody (Cat # PAB3350).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKA polyclonal antibody (Cat # PAB3350) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1606
  • Protein Accession#:
  • NP_958853;P23743
  • Gene Name:
  • DGKA
  • Gene Alias:
  • DAGK,DAGK1,DGK-alpha,MGC12821,MGC42356
  • Gene Description:
  • diacylglycerol kinase, alpha 80kDa
  • Gene Summary:
  • The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
  • Other Designations:
  • diacylglycerol kinase alpha,diacylglycerol kinase, alpha (80kD)
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