DGKI polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The DGKI polyclonal antibody (Cat # PAB3341) is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKI polyclonal antibody (Cat # PAB3341) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DGKI.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DGKI.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The DGKI polyclonal antibody (Cat # PAB3341) is used in Western blot to detect DGKI in mouse cerebellum tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKI polyclonal antibody (Cat # PAB3341) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — DGKI
Entrez GeneID
9162Protein Accession#
O75912Gene Name
DGKI
Gene Alias
DGK-IOTA
Gene Description
diacylglycerol kinase, iota
Omim ID
604072Gene Ontology
HyperlinkGene Summary
This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Evaluation of human diacylglycerol kinase(iota), DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q.
Bowne SJ, Sullivan LS, Ding L, Traer E, Prescott SM, Birch DG, Kennan A, Humphries P, Daiger SP.
Molecular Vision 2000 Feb; 6:6.
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The cloning and characterization of a novel human diacylglycerol kinase, DGKiota.
Ding L, Traer E, McIntyre TM, Zimmerman GA, Prescott SM.
The Journal of Biological Chemistry 1998 Dec; 273(49):32746.
Application：WB-Tr, Monkey, COS-7 cells.
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Evaluation of human diacylglycerol kinase(iota), DGKI, a homolog of Drosophila rdgA, in inherited retinopathy mapping to 7q.
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