DGKQ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NCI-H460 cell lysate (35 ug/lane) with DGKQ polyclonal antibody (Cat # PAB3339).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKQ polyclonal antibody (Cat # PAB3339) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DGKQ.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DGKQ.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NCI-H460 cell lysate (35 ug/lane) with DGKQ polyclonal antibody (Cat # PAB3339).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with DGKQ polyclonal antibody (Cat # PAB3339) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
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Gene Info — DGKQ
Entrez GeneID
1609Protein Accession#
P52824Gene Name
DGKQ
Gene Alias
DAGK, DAGK4, DAGK7
Gene Description
diacylglycerol kinase, theta 110kDa
Omim ID
601207Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq
Other Designations
diacylglycerol kinase (110kD)|diacylglycerol kinase, delta (110kD)|diacylglycerol kinase, theta|diacylglycerol kinase, theta (110kD)
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Interactome
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Pathway
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Publication Reference
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Chromosomal localization of three mouse diacylglycerol kinase (DAGK) genes: genes sharing sequence homology to the Drosophila retinal degeneration A (rdgA) gene.
Pilz A, Schaap D, Hunt D, Fitzgibbon J.
Genomics 1995 Apr; 26(3):599.
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Chromosomal localization of three mouse diacylglycerol kinase (DAGK) genes: genes sharing sequence homology to the Drosophila retinal degeneration A (rdgA) gene.
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