WEE1 polyclonal antibody
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More Files
- More Functions
Western Blot
The WEE1 polyclonal antibody (Cat # PAB3322) is used in Western blot to detect WEE1 in Jurkat cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WEE1 polyclonal antibody (Cat # PAB3322) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of WEE1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human WEE1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The WEE1 polyclonal antibody (Cat # PAB3322) is used in Western blot to detect WEE1 in Jurkat cell lysate (Lane 1) and mouse liver tissue lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WEE1 polyclonal antibody (Cat # PAB3322) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — WEE1
Entrez GeneID
7465Protein Accession#
P30291Gene Name
WEE1
Gene Alias
DKFZp686I18166, FLJ16446, WEE1A, WEE1hu
Gene Description
WEE1 homolog (S. pombe)
Omim ID
193525Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq
Other Designations
WEE1+ homolog|wee1 tyrosine kinase|wee1-like protein kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Human wee1 kinase is directly transactivated by and increased in association with c-Fos/AP-1: rheumatoid synovial cells overexpressing these genes go into aberrant mitosis.
Kawasaki H, Komai K, Nakamura M, Yamamoto E, Ouyang Z, Nakashima T, Morisawa T, Hashiramoto A, Shiozawa K, Ishikawa H, Kurosaka M, Shiozawa S.
Oncogene 2003 Oct; 22(44):6839.
Application：WB-Ce, Human, Synovial cells.
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Inhibition of proteasome-dependent degradation of Wee1 in G2-arrested Hep3B cells by TGF beta 1.
Hashimoto O, Ueno T, Kimura R, Ohtsubo M, Nakamura T, Koga H, Torimura T, Uchida S, Yamashita K, Sata M.
Molecular Carcinogenesis 2003 Apr; 36(4):171.
Application：WB, Human, Hep-3B, Huh7 cells.
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Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis.
Yuan H, Xie YM, Chen IS.
Journal of Virology 2003 Feb; 77(3):2063.
Application：WB, Human, HeLa cells.
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Human wee1 kinase is directly transactivated by and increased in association with c-Fos/AP-1: rheumatoid synovial cells overexpressing these genes go into aberrant mitosis.
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