TLK1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TLK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TLK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of TLK1 polyclonal antibody (Cat # PAB3318) in HL-60 cell lysate. TLK1 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TLK1 polyclonal antibody (Cat # PAB3318) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — TLK1
Entrez GeneID
9874Protein Accession#
Q9UKI8Gene Name
TLK1
Gene Alias
KIAA0137, PKU-beta
Gene Description
tousled-like kinase 1
Omim ID
608438Gene Ontology
HyperlinkGene Summary
The Tousled-like kinases, first described in Arabidopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly.[supplied by OMIM
Other Designations
SNARE protein kinase SNAK|serine threonine protein kinase
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Interactome
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Publication Reference
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Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
Krause DR, Jonnalagadda JC, Gatei MH, Sillje HH, Zhou BB, Nigg EA, Khanna K.
Oncogene 2003 Sep; 22(38):5927.
Application:IP, WB-Tr, Human, AT1ABR, C2ABR, HCT-115, L3 cells.
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Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex assembly.
Cabaniols JP, Ravichandran V, Roche PA.
Molecular Biology of the Cell 1999 Dec; 10(12):4033.
Application:IP, Human, HeLa cells.
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Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication.
Sillje HH, Takahashi K, Tanaka K, Van Houwe G, Nigg EA.
The EMBO Journal 1999 Oct; 18(20):5691.
Application:IF, IP, KA, WB-Ce, WB-Re, Human, HeLa cells, Recombinant protein, U2-OS cells.
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Suppression of Tousled-like kinase activity after DNA damage or replication block requires ATM, NBS1 and Chk1.
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