TP53RK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The TP53RK polyclonal antibody (Cat # PAB3315) is used in Western blot to detect TP53Rk in mouse thymus tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TP53RK polyclonal antibody (Cat # PAB3315) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TP53RK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human TP53RK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The TP53RK polyclonal antibody (Cat # PAB3315) is used in Western blot to detect TP53Rk in mouse thymus tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with TP53RK polyclonal antibody (Cat # PAB3315) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — TP53RK
Entrez GeneID
112858Protein Accession#
PRPK_HUMANGene Name
TP53RK
Gene Alias
BUD32, C20orf64, Nori-2, Nori-2p, PRPK, dJ101A2
Gene Description
TP53 regulating kinase
Omim ID
608679Gene Ontology
HyperlinkOther Designations
OTTHUMP00000108264|p53-related protein kinase
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Interactome
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Disease
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Publication Reference
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Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein.
Miyoshi A, Kito K, Aramoto T, Abe Y, Kobayashi N, Ueda N.
Biochemical and Biophysical Research Communications 2003 Apr; 303(2):399.
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Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes.
Abe Y, Matsumoto S, Wei S, Nezu K, Miyoshi A, Kito K, Ueda N, Shigemoto K, Hitsumoto Y, Nikawa J, Enomoto Y.
The Journal of Biological Chemistry 2001 Sep; 276(47):44003.
Application：WB-Ce, WB-Tr, Human, COS-7, HEK 293T, MDA-MB-231, MIA PaCa-2, PANC-1 cells.
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Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein.
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