PEBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
(LEFT) The PEBP1 polyclonal antibody (Cat # PAB3307) is used in Western blot to detect PEBP1 in HeLa cell lysate.
(RIGHT) Western blot analysis of PEBP1 polyclonal antibody (Cat # PAB3307) in Y-79 cell line lysate (35 ug/lane).
PEBP1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PEBP1 polyclonal antibody (Cat # PAB3307) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PEBP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PEBP1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
(LEFT) The PEBP1 polyclonal antibody (Cat # PAB3307) is used in Western blot to detect PEBP1 in HeLa cell lysate.
(RIGHT) Western blot analysis of PEBP1 polyclonal antibody (Cat # PAB3307) in Y-79 cell line lysate (35 ug/lane).
PEBP1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PEBP1 polyclonal antibody (Cat # PAB3307) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PEBP1
Entrez GeneID
5037Protein Accession#
NP_002558;P30086Gene Name
PEBP1
Gene Alias
HCNP, PBP, PEBP, RKIP
Gene Description
phosphatidylethanolamine binding protein 1
Omim ID
604591Gene Ontology
HyperlinkOther Designations
Raf kinase inhibitory protein|prostatic binding protein
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Interactome
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Publication Reference
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Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein.
Tohdoh N, Tojo S, Agui H, Ojika K.
Brain Research 1995 Jun; 30(2):381.
Application：IHC, WB-Ti, Rat, Rat medial septal tissues.
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Amino acid sequence of the Homo sapiens brain 21-23-kDa protein (neuropolypeptide h3), comparison with its counterparts from Rattus norvegicus and Bos taurus species, and expression of its mRNA in different tissues.
Seddiqi N, Bollengier F, Alliel PM, Perin JP, Bonnet F, Bucquoy S, Jolles P, Schoentgen F.
Journal of Molecular Evolution 1994 Dec; 39(6):655.
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A human cDNA sequence homologue of bovine phosphatidylethanolamine-binding protein.
Hori N, Chae KS, Murakawa K, Matoba R, Fukushima A, Okubo K, Matsubara K.
Gene 1994 Mar; 140(2):293.
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Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein.
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