PACSIN3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PACSIN3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PACSIN3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The PACSIN3 polyclonal antibody (Cat # PAB3303) is used in Western blot to detect PACSIN3 in mouse brain tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PACSIN3 polyclonal antibody (Cat # PAB3303) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PACSIN3
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Interactome
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Publication Reference
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PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family.
Sumoy L, Pluvinet R, Andreu N, Estivill X, Escarceller M.
Gene 2001 Jan; 262(1-2):199.
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All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis.
Modregger J, Ritter B, Witter B, Paulsson M, Plomann M.
Journal of Cell Science 2000 Dec; 113Pt 24:4511.
Application:IF, WB-Ti, Mouse, Brain, Heart, Liver, Lung, Kidney, Muscle, Spleen, Testis, Thymus, Uterus, C2F3 myotubes.
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Interaction of the metalloprotease disintegrins MDC9 and MDC15 with two SH3 domain-containing proteins, endophilin I and SH3PX1.
Howard L, Nelson KK, Maciewicz RA, Blobel CP.
The Journal of Biological Chemistry 1999 Oct; 274(44):31693.
Application:WB-Ce, WB-Tr, Human, Monkey, COS-7, HEK 293, HeLa cells.
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PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family.
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