PACSIN1 polyclonal antibody
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More Files
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Western Blot
Western blot analysis of PACSIN1 polyclonal antibody (Cat # PAB3299) in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) . PACSIN1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PACSIN1 polyclonal antibody (Cat # PAB3299) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PACSIN1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PACSIN1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PACSIN1 polyclonal antibody (Cat # PAB3299) in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2) . PACSIN1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PACSIN1 polyclonal antibody (Cat # PAB3299) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PACSIN1
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Interactome
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Disease
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Publication Reference
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PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family.
Sumoy L, Pluvinet R, Andreu N, Estivill X, Escarceller M.
Gene 2001 Jan; 262(1-2):199.
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PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family.
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