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Last updated: 2017/12/10
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NME2 polyclonal antibody

  • Catalog # : PAB3291
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of NME2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NME2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunofluorescence (1:10-50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in mouse brain tissue lysate. NME2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in NCI-H460 cell line lysates (35 ug/lane). NME2 (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME2 polyclonal antibody (Cat # PAB3291) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Immunofluorescence
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Gene Information
  • Entrez GeneID:
  • 4831
  • Gene Name:
  • NME2
  • Gene Alias:
  • MGC111212,NDPK-B,NDPKB,NM23-H2,NM23B,puf
  • Gene Description:
  • non-metastatic cells 2, protein (NM23B) expressed in
  • Gene Summary:
  • Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
  • Other Designations:
  • NDP kinase B,OTTHUMP00000174727,OTTHUMP00000174728,OTTHUMP00000174774,OTTHUMP00000174775,OTTHUMP00000174776,c-myc transcription factor,non-metastatic cells 2, protein (NM23) expressed in
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