NME2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in mouse brain tissue lysate. NME2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot (Cell lysate)
Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in NCI-H460 cell line lysates (35 ug/lane). NME2 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME2 polyclonal antibody (Cat # PAB3291) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NME2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NME2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in mouse brain tissue lysate. NME2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Western Blot (Cell lysate)
Western blot analysis of NME2 polyclonal antibody (Cat # PAB3291) in NCI-H460 cell line lysates (35 ug/lane). NME2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NME2 polyclonal antibody (Cat # PAB3291) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
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Gene Info — NME2
Entrez GeneID
4831Protein Accession#
P22392Gene Name
NME2
Gene Alias
MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf
Gene Description
non-metastatic cells 2, protein (NM23B) expressed in
Omim ID
156491Gene Ontology
HyperlinkGene Summary
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq
Other Designations
NDP kinase B|OTTHUMP00000174727|OTTHUMP00000174728|OTTHUMP00000174774|OTTHUMP00000174775|OTTHUMP00000174776|c-myc transcription factor|non-metastatic cells 2, protein (NM23) expressed in
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Interactome
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Pathway
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Publication Reference
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Nm23/NDP kinases in human male germ cells: role in spermiogenesis and sperm motility?
Munier A, Serres C, Kann ML, Boissan M, Lesaffre C, Capeau J, Fouquet JP, Lacombe ML.
Experimental Cell Research 2003 Oct; 289(2):295.
Application：ELISA, IEM, IF, IHC-P, WB, Human, Spermatozoa, Testis biopsies.
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Human brain nucleoside diphosphate kinase activity is decreased in Alzheimer's disease and Down syndrome.
Kim SH, Fountoulakis M, Cairns NJ, Lubec G.
Biochemical and Biophysical Research Communications 2002 Aug; 296(4):970.
Application：WB-Ti, Human, Brain.
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Expression of cell surface NM23 proteins of human leukemia cell lines of various cellular lineage and differentiation stages.
Okabe-Kado J, Kasukabe T, Honma Y.
Leukemia Research 2002 Jun; 26(6):569.
Application：CDC, Flow Cyt, Human, HL60, HEL, K562 cells.
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Nm23/NDP kinases in human male germ cells: role in spermiogenesis and sperm motility?
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