NEK9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NEK9.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The NEK9 polyclonal antibody (Cat # PAB3289) is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK9 polyclonal antibody (Cat # PAB3289) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — NEK9
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Interactome
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Disease
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Publication Reference
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Nek9, a novel FACT-associated protein, modulates interphase progression.
Tan BC, Lee SC.
The Journal of Biological Chemistry 2004 Mar; 279(10):9321.
Application:IF, IP-WB, WB-Ce, Human, HeLa cells.
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A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.
Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J.
The Journal of Biological Chemistry 2003 Sep; 278(37):34897.
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Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.
Roig J, Mikhailov A, Belham C, Avruch J.
Genes & Development 2002 Jul; 16(13):1640.
Application:IF, IP, WB-Ce, Human, HeLa cells.
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Nek9, a novel FACT-associated protein, modulates interphase progression.
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