Western blot analysis of CEM cell lysate (35 ug/lane) with NEK3 polyclonal antibody (Cat # PAB3285).
Western Blot (Transfected lysate)
Western blot analysis of NEK3 (arrow) using NEK3 polyclonal antibody (Cat # PAB3285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK3 gene (Lane 2).
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
NIMA-related kinase 3,glycogen synthase A kinase,hydroxyalkyl-protein kinase,phosphorylase B kinase kinase,serine/threonine-protein kinase NEK3