NEK3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CEM cell lysate (35 ug/lane) with NEK3 polyclonal antibody (Cat # PAB3285).
Western Blot (Transfected lysate)
Western blot analysis of NEK3 (arrow) using NEK3 polyclonal antibody (Cat # PAB3285).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK3 gene (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human NEK3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CEM cell lysate (35 ug/lane) with NEK3 polyclonal antibody (Cat # PAB3285).Western Blot (Transfected lysate)
Western blot analysis of NEK3 (arrow) using NEK3 polyclonal antibody (Cat # PAB3285).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK3 gene (Lane 2). -
Gene Info — NEK3
Entrez GeneID
4752Protein Accession#
NEK3_HUMAN;NP_002489Gene Name
NEK3
Gene Alias
HSPK36, MGC29949
Gene Description
NIMA (never in mitosis gene a)-related kinase 3
Omim ID
604044Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene
Other Designations
NIMA-related kinase 3|glycogen synthase A kinase|hydroxyalkyl-protein kinase|phosphorylase B kinase kinase|serine/threonine-protein kinase NEK3
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Interactome
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Disease
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Publication Reference
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Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3).
Kimura M, Okano Y.
Cytogenetics and Cell Genetics 2001 Dec; 95(3-4):177.
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Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.
Schultz SJ, Fry AM, Sutterlin C, Ried T, Nigg EA.
Cell growth & Differentiation: the Molecular Biology Journal of the American Association for Cancer 1994 Jun; 5(6):625.
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Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans.
Schultz SJ, Nigg EA.
Cell Growth & Differentiation 1993 Oct; 4(10):821.
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Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3).
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