NEK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human NEK2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunofluorescence (1:25)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NEK2 polyclonal antibody (Cat # PAB3284) in mouse lung tissue lysate. NEK2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
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Gene Info — NEK2
Entrez GeneID
4751Protein Accession#
NEK2_HUMANGene Name
NEK2
Gene Alias
HsPK21, NEK2A, NLK1
Gene Description
NIMA (never in mitosis gene a)-related kinase 2
Omim ID
604043Gene Ontology
HyperlinkOther Designations
OTTHUMP00000035216
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Interactome
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Disease
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Publication Reference
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Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.
Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH.
The Journal of Biological Chemistry 2002 Dec; 277(51):49408.
Application:IP-WB, WB-Ce, Human, Insect, T24, Sf9 cells.
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A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41.
Schutte BC, Bjork BC, Coppage KB, Malik MI, Gregory SG, Scott DJ, Brentzell LM, Watanabe Y, Dixon MJ, Murray JC.
Genome Research 2000 Jan; 10(1):81.
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Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation.
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