NEK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The NEK1 polyclonal antibody (Cat # PAB3282) is used in Western blot to detect NEK1 in CHO cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK1 polyclonal antibody (Cat # PAB3282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NEK1.
Host
Rabbit
Reactivity
Hamster, Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The NEK1 polyclonal antibody (Cat # PAB3282) is used in Western blot to detect NEK1 in CHO cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEK1 polyclonal antibody (Cat # PAB3282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — NEK1
Entrez GeneID
4750Protein Accession#
NEK1_HUMAN;Q96PY6Gene Name
NEK1
Gene Alias
DKFZp686D06121, DKFZp686K12169, KIAA1901, MGC138800, NY-REN-55
Gene Description
NIMA (never in mitosis gene a)-related kinase 1
Omim ID
604588Gene Ontology
HyperlinkOther Designations
protein-serine/threonine kinase|serine/threonine-protein kinase Nek1
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Interactome
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Publication Reference
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Revealing A-Raf functions through its interactome.
Zhang XY, Guo H, Han B, Zhang XM, Huang Y, Yang Y, Liu Y, Guo XX, Hao Q, An S, Tian-RuiXu.
Biochimica et Biophysica Acta 2018 Aug; 1866(8):849.
Application：WB-Tr, Human, Flp-In T-Rex HEK293 cells.
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The master cell cycle regulator APC-Cdc20 regulates ciliary length and disassembly of the primary cilium.
Wang W, Wu T, Kirschner MW.
ELife 2014 Aug; 3:e03083.
Application：WB, Human, hTERT-RPE1 cells.
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Nek1 and TAZ Interact to Maintain Normal Levels of Polycystin 2.
Yim H, Sung CK, You J, Tian Y, Benjamin T.
Journal of the American Society of Nephrology 2011 May; 22(5):832.
Application：WB, Mouse, Fibroblast cells.
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Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1.
Surpili MJ, Delben TM, Kobarg J.
Biochemistry 2003 Dec; 42(51):15369.
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Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jager D, Jager E, Knuth A, Chen YT, Old LJ.
International Journal of Cancer 1999 Nov; 83(4):456.
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Revealing A-Raf functions through its interactome.
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