MLKLAK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MLKLAK polyclonal antibody (Cat # PAB3277) in HepG2 cell line lysate (35 ug/lane). MLKLAK (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution). This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MLKLAK polyclonal antibody (Cat # PAB3277) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MLKLAK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MLKLAK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MLKLAK polyclonal antibody (Cat # PAB3277) in HepG2 cell line lysate (35 ug/lane). MLKLAK (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution). This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MLKLAK polyclonal antibody (Cat # PAB3277) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — ZAK
Entrez GeneID
51776Protein Accession#
Q9NYL2-2;NP_598407Gene Name
ZAK
Gene Alias
AZK, MLK7, MLT, MLTK, MRK, mlklak
Gene Description
sterile alpha motif and leucine zipper containing kinase AZK
Omim ID
609479Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
MLK-like mitogen-activated protein triple kinase|MLK-related kinase|cervical cancer suppressor gene 4 protein|leucine zipper- and sterile alpha motif-containing kinase|mitogen-activated protein kinase kinase kinase MLT|mixed lineage kinase 7|mixed lineage
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Interactome
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Pathway
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Disease
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Publication Reference
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Oncogenic kinase signalling.
Blume-Jensen P, Hunter T.
Nature 2001 May; 411(6835):355.
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Phosphoinositide 3-kinase signalling pathways.
Cantrell DA.
Journal of Cell Science 2001 Apr; 114(Pt 8):1439.
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The RET proto-oncogene in human cancers.
Jhiang SM.
Oncogene 2000 Nov; 19(49):5590.
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Oncogenic kinase signalling.
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