NUAK1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NUAK1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human NUAK1.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of NUAK1 polyclonal antibody (Cat # PAB3274) in mouse brain tissue lysate. NUAK1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with NUAK1 polyclonal antibody (Cat # PAB3274) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — NUAK1
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Interactome
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Disease
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Publication Reference
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ARK5 is a tumor invasion-associated factor downstream of Akt signaling.
Suzuki A, Lu J, Kusakai G, Kishimoto A, Ogura T, Esumi H.
Molecular and Cellular Biology 2004 Apr; 24(8):3526.
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ARK5 suppresses the cell death induced by nutrient starvation and death receptors via inhibition of caspase 8 activation, but not by chemotherapeutic agents or UV irradiation.
Suzuki A, Kusakai G, Kishimoto A, Lu J, Ogura T, Esumi H.
Oncogene 2003 Sep; 22(40):6177.
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Identification of a novel protein kinase mediating Akt survival signaling to the ATM protein.
Suzuki A, Kusakai G, Kishimoto A, Lu J, Ogura T, Lavin MF, Esumi H.
The Journal of Biological Chemistry 2003 Jan; 278(1):48.
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ARK5 is a tumor invasion-associated factor downstream of Akt signaling.
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