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Last updated: 2016/10/23
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EIF2AK1 polyclonal antibody

  • Catalog # : PAB3272
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EIF2AK1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EIF2AK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of EIF2AK1 polyclonal antibody (Cat # PAB3272) in A-375 cell lysate. EIF2AK1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with EIF2AK1 polyclonal antibody (Cat # PAB3272) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • EIF2AK1
  • Gene Alias:
  • HCR,HRI,KIAA1369
  • Gene Description:
  • eukaryotic translation initiation factor 2-alpha kinase 1
  • Gene Summary:
  • The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • heme regulated initiation factor 2 alpha kinase,heme sensitive initiation factor 2a kinase,heme-regulated inhibitor,heme-regulated initiation factor 2-alpha kinase,heme-regulated repressor
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