PIP5K1B polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIP5K1B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PIP5K1B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PIP5K1B polyclonal antibody (Cat # PAB3233) in human placenta tissue lysate. PIP5K1B (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIP5K1B polyclonal antibody (Cat # PAB3233) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — PIP5K1B
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Interactome
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Pathway
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Disease
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Publication Reference
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PIP5KIβ Selectively Modulates Apical Endocytosis in Polarized Renal Epithelial Cells.
Szalinski CM, Guerriero CJ, Ruiz WG, Docter BE, Rbaibi Y, Pastor-Soler NM, Apodaca G, Puthenveedu MA, Weisz OA.
PLoS One 2013 Jan; 8(1):e53790.
Application:IF, Mouse, Rat, Mouse cortical collecting duct cell, Rat kidney cortex.
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Oncogenic kinase signalling.
Blume-Jensen P, Hunter T.
Nature 2001 May; 411(6835):355.
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Phosphoinositide 3-kinase signalling pathways.
Cantrell DA.
Journal of Cell Science 2001 Apr; 114(Pt 8):1439.
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The RET proto-oncogene in human cancers.
Jhiang SM.
Oncogene 2000 Nov; 19(49):5590.
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PIP5KIβ Selectively Modulates Apical Endocytosis in Polarized Renal Epithelial Cells.
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