PI4K2B polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PI4K2B polyclonal antibody (Cat # PAB3229) in HL-60 cell lysate. PI4K2B (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PI4K2B polyclonal antibody (Cat # PAB3229) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PI4K2B polyclonal antibody (Cat # PAB3229) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PI4K2B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PI4K2B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PI4K2B polyclonal antibody (Cat # PAB3229) in HL-60 cell lysate. PI4K2B (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PI4K2B polyclonal antibody (Cat # PAB3229) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PI4K2B polyclonal antibody (Cat # PAB3229) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PI4K2B
Entrez GeneID
55300Protein Accession#
NP_060793Gene Name
PI4K2B
Gene Alias
FLJ11105, PI4KIIB, PIK42B
Gene Description
phosphatidylinositol 4-kinase type 2 beta
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4,5-bisphosphate synthesis (Wei et al., 2002 [PubMed 12324459]).[supplied by OMIM
Other Designations
2610042N09Rik|4933409G22Rik|OTTHUMP00000158714|phosphatidylinositol 4-kinase type-II beta
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Interactome
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Disease
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Publication Reference
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Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments.
Balla A, Tuymetova G, Barshishat M, Geiszt M, Balla T.
The Journal of Biological Chemistry 2002 May; 277(22):20041.
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Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments.
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