Western blot analysis of PIK3R1 polyclonal antibody (Cat # PAB3220) in CEM and Hela cell line lysates (35 ug/lane). PIK3R1 (arrow) was detected using the purified polyclonal antibody (1 : 63 dilution).
Western Blot (Transfected lysate)
Western blot analysis of PIK3R1 polyclonal antibody (Cat # PAB3220) in 293 cell line lysates transiently transfected with the PIK3R1 gene (3 ug/lane). PI3KR1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PIK3R1 polyclonal antibody (Cat # PAB3220) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq