PIK3C2B polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PIK3C2B polyclonal antibody (Cat # PAB3207) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PIK3C2B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PIK3C2B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with PIK3C2B polyclonal antibody (Cat # PAB3207) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — PIK3C2B
Entrez GeneID
5287Protein Accession#
O00750Gene Name
PIK3C2B
Gene Alias
C2-PI3K, DKFZp686G16234
Gene Description
phosphoinositide-3-kinase, class 2, beta polypeptide
Omim ID
602838Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq
Other Designations
OTTHUMP00000034333|PI3K-C2beta|PTDINS-3-kinase C2 beta|phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity.
Arcaro A, Volinia S, Zvelebil MJ, Stein R, Watton SJ, Layton MJ, Gout I, Ahmadi K, Downward J, Waterfield MD.
The Journal of Biological Chemistry 1998 Dec; 273(49):33082.
Application：WB-Tr, Human, HEK 293 cells.
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Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K.
Brown RA, Ho LK, Weber-Hall SJ, Shipley JM, Fry MJ.
Biochemical and Biophysical Research Communications 1997 Apr; 233(2):537.
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Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity.
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