The DNA sequence and biological annotation of human chromosome 1. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, ClarkeNature. 2006 May 18;441(7091):315-21.
Western Blot (Transfected lysate)
Western blot analysis of MLK4 (arrow) using rabbit MLK4 polyclonal antibody (Cat # PAB3199). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLK4 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 polyclonal antibody (Cat # PAB3199) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.