MLK4 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of MLK4 (arrow) using rabbit MLK4 polyclonal antibody (Cat # PAB3199). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLK4 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 polyclonal antibody (Cat # PAB3199) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MLK4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MLK4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of MLK4 (arrow) using rabbit MLK4 polyclonal antibody (Cat # PAB3199). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MLK4 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 polyclonal antibody (Cat # PAB3199) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — KIAA1804
Entrez GeneID
84451Protein Accession#
NP_115811;Q5TCX8Gene Name
KIAA1804
Gene Alias
MLK4, RP5-862P8.2, dJ862P8.3
Gene Description
mixed lineage kinase 4
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000036006
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Interactome
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Disease
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Publication Reference
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The DNA sequence and biological annotation of human chromosome 1.
Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke
Nature 2006 May; 441(7091):315.
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The DNA sequence and biological annotation of human chromosome 1.
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