Products

Western Blot (Cell lysate)

Western blot analysis of MAP4K3 polyclonal antibody (Cat # PAB3198) in HeLa cell lysate. Dilution for MAP4K3 polyclonal antibody (Cat # PAB3198) was 1 : 100 ; dilution for secondary goat anti-rabbit-HRP was 1 : 7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K3 polyclonal antibody (Cat # PAB3198) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Entrez GeneID

8491

Protein Accession#

Q8IVH8

Gene Name

MAP4K3

Gene Alias

GLK, MAPKKKK3, RAB8IPL1

Gene Description

mitogen-activated protein kinase kinase kinase kinase 3

Omim ID

604921

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3). [provided by RefSeq

Other Designations

germinal center kinase-like kinase|germinal center kinase-related protein kinase