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Last updated: 2016/10/23
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MAP4K3 polyclonal antibody

  • Catalog # : PAB3198
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of MAP4K3.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP4K3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of MAP4K3 polyclonal antibody (Cat # PAB3198) in HeLa cell lysate. Dilution for MAP4K3 polyclonal antibody (Cat # PAB3198) was 1 : 100 ; dilution for secondary goat anti-rabbit-HRP was 1 : 7000. A chemiluminescent kit was used for development of the Western blot. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K3 polyclonal antibody (Cat # PAB3198) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 8491
  • Gene Name:
  • MAP4K3
  • Gene Alias:
  • Gene Description:
  • mitogen-activated protein kinase kinase kinase kinase 3
  • Gene Summary:
  • This gene encodes a member of the Ste20 family of serine/threonine protein kinases. The protein belongs to the subfamily that consists of members, such as germinal center kinase (GCK), that are characterized by an N-terminal catalytic domain and C-terminal regulatory domain. The kinase activity of the encoded protein can be stimulated by UV radiation and tumor necrosis factor-alpha. The protein specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway. Evidence suggests that it functions upstream of mitogen-activated protein kinase kinase kinase 1 (MEKK1). This gene previously was referred to as RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3). [provided by RefSeq
  • Other Designations:
  • germinal center kinase-like kinase,germinal center kinase-related protein kinase
  • Gene Pathway
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