MAP4K1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAP4K1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MAP4K1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MAP4K1 polyclonal antibody (Cat # PAB3188) in A-375 cell lysate. MAP4K1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K1 polyclonal antibody (Cat # PAB3188) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — MAP4K1
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Interactome
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Pathway
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Publication Reference
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Hematopoietic progenitor kinase 1 (HPK1) negatively regulates prostaglandin E2-induced fos gene transcription.
Sawasdikosol S, Russo KM, Burakoff SJ.
Blood 2003 May; 101(9):3687.
Application:WB-Tr, Human, Jurkat T cells.
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Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH.
Genes & Development 1996 Sep; 10(18):2251.
Application:IP, Human, Daudi cells.
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Hematopoietic progenitor kinase 1 (HPK1) negatively regulates prostaglandin E2-induced fos gene transcription.
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