POU2F3 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human POU2F3.
Sequence
LESMHTDIKMSGDVADSTDARSTLSQVEPGNDRNGLDFNRQIKTEDLSDSLQQTLSHRPCHLSQGPAMMSGNQMSGLNASPCQDMASLHPLQQLVLVPGHLQSVSQFLLSQTQP
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts to POU2F3.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:200 - 1:500)
Western Blot (0.04-0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of control (vector only transfected HEK293T lysate) and POU2F3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry
Immunohistochemical staining of human kidney shows no positivity in tubules.Immunohistochemistry
Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells.Immunohistochemistry
Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.Immunohistochemistry
Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes. -
Gene Info — POU2F3
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Interactome
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Disease
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