AQP4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of human brain tissue.
Immunohistochemistry
Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in astrocytes.
Immunohistochemistry
Immunohistochemical staining of human cerebellum shows strong membranous positivity in astrocytes, mainly in the molecular layer.
Immunohistochemistry
Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.
Immunohistochemistry
Immunohistochemical staining of human liver shows no positivity in hepatocytes, as expected.
Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Immunofluorescence
Immunofluorescent staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human AQP4.
Sequence
CPDVEFKRRFKEAFSKAAQQTKGSYMEVEDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody reacts to AQP4.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (0.25-2 ug/mL)
Immunohistochemistry (1:2500 - 1:5000)
Western Blot (0.04-0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of human brain tissue.Immunohistochemistry
Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in astrocytes.Immunohistochemistry
Immunohistochemical staining of human cerebellum shows strong membranous positivity in astrocytes, mainly in the molecular layer.Immunohistochemistry
Immunohistochemical staining of human stomach shows strong membranous positivity in glandular cells.Immunohistochemistry
Immunohistochemical staining of human liver shows no positivity in hepatocytes, as expected.Immunofluorescence
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.Immunofluorescence
Immunofluorescent staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer. -
Gene Info — AQP4
Entrez GeneID
361Gene Name
AQP4
Gene Alias
HMIWC2, MGC22454, MIWC
Gene Description
aquaporin 4
Omim ID
600308Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
aquaporin type4|aquaporin-4|mercurial-insensitive water channel
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Interactome
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Disease
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