GSPT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using GSPT1 polyclonal antibody (Cat # PAB31821). Loading control: Anti-PARP1.
Immunohistochemistry
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry
Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Immunohistochemistry
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts and Leydig cells.
Immunohistochemistry
Immunohistochemical staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.
Immunofluorescence
Immunofluorescent staining of human cell line HeLa shows localization to cytosol and vesicles.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human GSPT1.
Sequence
GGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVS
Host
Rabbit
Reactivity
Human
Specificity
This antibody reacts to GSPT1.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (0.25-2 ug/mL)
Immunohistochemistry (1:200 - 1:500)
Western Blot (0.04-0.4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using GSPT1 polyclonal antibody (Cat # PAB31821). Loading control: Anti-PARP1.Immunohistochemistry
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.Immunohistochemistry
Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.Immunohistochemistry
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts and Leydig cells.Immunohistochemistry
Immunohistochemical staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.Immunofluorescence
Immunofluorescent staining of human cell line HeLa shows localization to cytosol and vesicles. -
Gene Info — GSPT1
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Interactome
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