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Last updated: 2022/5/15

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GSPT1 polyclonal antibody 

  • Catalog # : PAB31821
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Rabbit monoclonal antibody raised against recombinant human GSPT1.
  • Sequence:
  • GGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVS
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts to GSPT1.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • Immunofluorescence (0.25-2 ug/mL)
    Immunohistochemistry (1:200 - 1:500)
    Western Blot (0.04-0.4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH7.2 (40% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using GSPT1 polyclonal antibody (Cat # PAB31821). Loading control: Anti-PARP1.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in seminiferous ducts and Leydig cells.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.
  • Immunohistochemistry
  • Immunohistochemistry
  • Immunohistochemical staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescent staining of human cell line HeLa shows localization to cytosol and vesicles.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2935
  • Gene Name:
  • GSPT1
  • Gene Alias:
  • 551G9.2,ETF3A,FLJ38048,FLJ39067,GST1,eRF3a
  • Gene Description:
  • G1 to S phase transition 1
  • Other Designations:
  • G1 to S phase transition protein
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