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GFP polyclonal antibody (HRP) 

  • Catalog # : PAB31813
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Horseradish Peroxidase (HRP).
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Rabbit
  • Specificity:
  • wt, rGFP, eGFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-peroxidase and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.
  • Form:
  • Lyophilized
  • Conjugation:
  • HRP
  • Preparation Method:
  • This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Purification:
  • Affinity chromatography
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Immunohistochemistry (1:500-1:2000)
    Western Blot (1:2000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane: 50 ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: GFP polyclonal antibody (HRP) (Cat # PAB31813) was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20°C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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