GFP polyclonal antibody (FITC)
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More Files
- More Functions
Western Blot
Western blot analysis of Lane: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: GFP polyclonal antibody (FITC) (Cat # PAB31812) at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Fluorescein (FITC).
Immunogen
Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
Host
Rabbit
Specificity
wt, rGFP, eGFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-Fluorescein and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.
Form
Lyophilized
Conjugation
FITC
Preparation Method
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Purification
Affinity chromatography
Isotype
IgG
Conjugation Note
FP Value: 7.2 moles Fluorescein (FITC) per mole of IgG
Recommend Usage
ELISA (1:20000-1:40000)
Flow Cytometry (1:2000)
Immunofluorescence (1:500-1:2500)
Western Blot (1:10000-1:25000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: GFP polyclonal antibody (FITC) (Cat # PAB31812) at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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