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Last updated: 2021/8/8

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GFP polyclonal antibody (FITC) 

  • Catalog # : PAB31812
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant GFP. The antibody is conjugated with Fluorescein (FITC).
  • Immunogen:
  • Recombinant fusion protein corresponding to full length amino acid sequence (246 amino acids) derived from the jellyfish Aequorea victoria GFP.
  • Host:
  • Rabbit
  • Specificity:
  • wt, rGFP, eGFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, anti-Fluorescein and purified and partially purified green fluorescent protein (Aequorea victoria) serum. No reaction was observed against human, mouse and rat serum proteins.
  • Form:
  • Lyophilized
  • Conjugation:
  • FITC
  • Preparation Method:
  • This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Purification:
  • Affinity chromatography
  • Isotype:
  • IgG
  • Conjugation Note:
  • FP Value: 7.2 moles Fluorescein (FITC) per mole of IgG
  • Recommend Usage:
  • ELISA (1:20000-1:40000)
    Flow Cytometry (1:2000)
    Immunofluorescence (1:500-1:2500)
    Western Blot (1:10000-1:25000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: GFP polyclonal antibody (FITC) (Cat # PAB31812) at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Application Image
  • Immunofluorescence
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
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