His tag polyclonal antibody (HRP)
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Specification
Product Description
Rabbit polyclonal antibody raised against 6X His epitope tag peptide. The antibody is conjugated with Horseradish Peroxidase (HRP).
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Sequence
HHHHHH
Host
Rabbit
Specificity
This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Form
Lyophilized
Conjugation
HRP
Preparation Method
This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Purification
Affinity purification
Isotype
IgG
Recommend Usage
ELISA (1:5000)
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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