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Last updated: 2021/8/8

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His tag polyclonal antibody (Biotin) 

  • Catalog # : PAB31807
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against 6X His epitope tag peptide. The antibody is conjugated with Biotin.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to His tag.
  • Sequence:
  • HHHHHH
  • Host:
  • Rabbit
  • Specificity:
  • This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.? The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Preparation Method:
  • This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:2000 - 1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) sodium azide.
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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