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HA tag polyclonal antibody (HRP) 

  • Catalog # : PAB31803
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against HA epitope tag peptide. The antibody is conjugated with Horseradish peroxidase (HRP).
  • Immunogen:
  • A epitope tag peptide (conjugated with KLH) corresponding to YPYDVPDYA from hemagglutinin influenza.
  • Sequence:
  • YPYDVPDYA
  • Host:
  • Rabbit
  • Specificity:
  • This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
  • Form:
  • Lyophilized
  • Conjugation:
  • HRP
  • Preparation Method:
  • This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Recommend Usage:
  • ELISA (1:5000)
    Immunohistochemistry (1:500-1:2000)
    Western Blot (1:1000-1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) Gentamicin Sulfate). Do NOT add sodium azide!
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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