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HA tag polyclonal antibody (Biotin) 

  • Catalog # : PAB31802
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against HA epitope tag peptide. The antibody is conjugated with Biotin.
  • Immunogen:
  • A epitope tag peptide (conjugated with KLH) corresponding to YPYDVPDYA from hemagglutinin influenza.
  • Sequence:
  • YPYDVPDYA
  • Host:
  • Rabbit
  • Specificity:
  • This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-rabbit serum.
  • Form:
  • Lyophilized
  • Conjugation:
  • Biotin
  • Preparation Method:
  • This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
  • Purification:
  • Affinity purification
  • Isotype:
  • IgG
  • Conjugation Note:
  • FP Value: 10-20 moles Biotin per mole of IgG
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Immunohistochemistry (1:1000-1:5000)
    Western Blot (1:2000-1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
  • Storage Instruction:
  • Store vial at 4°C prior to restoration.
    After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
    Aliquot to avoid repeated freezing and thawing.
    Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western Blot analysis of recombinant transcription factor protein containing the HA epitope tag (1.0 ug) with HA tag polyclonal antibody (Biotin) (Cat # PAB31802) at 1:10000 working concentration.
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunohistochemistry
  • Enzyme-linked Immunoabsorbent Assay
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