Goat Fab Anti-Human IgG (H&L) secondary antibody (HRP)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Goat Fab polyclonal antibody raised against human IgG (H&L). The antibody is conjugated with Horseradish Peroxidase (HRP).
Immunogen
Human IgG whole molecule.
Host
Goat
Reactivity
Human
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-goat serum. No reaction was observed against anti-papain or anti-goat IgG F(c).
Form
Lyophilized
Conjugation
HRP
Purification
This antibody was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Isotype
IgG Fab
Recommend Usage
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 1.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com